Journal: Tissue Engineering. Part A

Article Title: Biodegradable Meshes Printed with Extracellular Matrix Proteins Support Micropatterned Hepatocyte Cultures

doi: 10.1089/ten.tea.2008.0265

Figure Lengend Snippet: Extracellular matrix protein microarray. (A) Diagram showing composition and position of all 64 unique compositions of collagen I, III, and IV; fibronectin (Fn); laminin (Ln); and Engelbreth-Holm-Swarm matrix (E). Number adjacent to each spot corresponds to the identity (ID) of the mixture in Table 1. Position of the pure Fn spot is indicated, and the box shows its nearest extracellular matrix (ECM) neighbors. (B) Diagram showing pattern of protein deposition for each of the six ECM proteins used to print the microarray. (C) Proteins were deposited in four replicates onto HydroGel microarray slides using a Virtek ChipWriter Pro robotic microarrayer.

Article Snippet: Position of the pure Fn spot is indicated, and the box shows its nearest extracellular matrix (ECM) neighbors. ( B ) Diagram showing pattern of protein deposition for each of the six ECM proteins used to print the microarray. ( C ) Proteins were deposited in four replicates onto HydroGel microarray slides using a Virtek ChipWriter Pro robotic microarrayer. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 ID Collagen I (C1) Collagen III (C3) Collagen IV (C4) Fibronectin (Fn) Laminin (Ln) EHS Matrix (E) No. of ECM proteins 1 Y N N N N N 1 2 N Y N N N N 3 N N Y N N N 4 N N N Y N N 5 N N N N Y N 6 N N N N N Y 7 Y Y N N N N 2 8 Y N Y N N N 9 Y N N Y N N 10 Y N N N Y N 11 Y N N N N Y 12 N Y Y N N N 13 N Y N Y N N 14 N Y N N Y N 15 N Y N N N Y 16 N N Y Y N N 17 N N Y N Y N 18 N N Y N N Y 19 N N N Y Y N 20 N N N Y N Y 21 N N N N Y Y 22 Y Y Y N N N 3 23 Y Y N Y N N 24 Y Y N N Y N 25 Y Y N N N Y 26 Y N Y Y N N 27 Y N Y N Y N 28 Y N Y N N Y 29 Y N N Y Y N 30 Y N N Y N Y 31 Y N N N Y Y 32 N Y Y Y N N 33 N Y Y N Y N 34 N Y Y N N Y 35 N Y N Y Y N 36 N Y N Y N Y 37 N Y N N Y Y 38 N N Y Y Y N 39 N N Y Y N Y 40 N N Y N Y Y 41 N N N Y Y Y 42 N N Y Y Y Y 4 43 N Y N Y Y Y 44 N Y Y N Y Y 45 N Y Y Y N Y 46 N Y Y Y Y N 47 Y N N Y Y Y 48 Y N Y N Y Y 49 Y N Y Y N Y 50 Y N Y Y Y N 51 Y Y N N Y Y 52 Y Y N Y N Y 53 Y Y N Y Y N 54 Y Y Y N N Y 55 Y Y Y N Y N 56 Y Y Y Y N N 57 N Y Y Y Y Y 5 58 Y N Y Y Y Y 59 Y Y N Y Y Y 60 Y Y Y N Y Y 61 Y Y Y Y N Y 62 Y Y Y Y Y N 63 N N N N N N 0 64 Y Y Y Y Y Y 6 Open in a separate window Components of Extracellular Matrix (ECM) Combinatorial Array

Techniques: Microarray

Journal: BMC Plant Biology

Article Title: Characterization of WRKY co-regulatory networks in rice and Arabidopsis

doi: 10.1186/1471-2229-9-120

Figure Lengend Snippet: Clustering of OsWRKY genes according to their expression profiles in the OsWRKYARRAY . The OsWRKYARRAY was constitued of 104 probesets representing all members of the rice WRKY gene family. The expression of the 104 OsWRKY genes was assessed upon inoculation with Magnaporthe oryzae isolate from rice (FR13), M. oryzae BR32 from wheat, M. grisea BR29 from crabgrass and upon application of osmotic stress (mannitol) in hydroponic conditions. Panel A T-test P-values (shown by a green - black gradient) of treated vs control of the corresponding ratios shown in Panel B. The range of log transformed P-values comprised values between 0.01 (green) and 1 (black). P-values lower than 0.01 were visualized as 0.01. Panel B log 2 (Treated/Control) ratio values (shown by a green - magenta gradient). Red boxes with capital letters from A to F highlight the presence of co-expressed WRKY gene clusters. A blue dot indicates a OsWRKY gene differentially-regulated in two different stress conditions; a yellow dot indicates a OsWRKY gene-differentially regulated only in one stress condition. See Table 1 for numeric values of differentially-regulated OsWRKY genes.

Article Snippet: The customized OsWRKYARRAY microarrays were produced using a Virtek ChipWriter Pro contact printing robot.

Techniques: Expressing, Transformation Assay

Journal: Tissue Engineering. Part A

Article Title: Biodegradable Meshes Printed with Extracellular Matrix Proteins Support Micropatterned Hepatocyte Cultures

doi: 10.1089/ten.tea.2008.0265

Figure Lengend Snippet: Extracellular matrix protein microarray. (A) Diagram showing composition and position of all 64 unique compositions of collagen I, III, and IV; fibronectin (Fn); laminin (Ln); and Engelbreth-Holm-Swarm matrix (E). Number adjacent to each spot corresponds to the identity (ID) of the mixture in Table 1. Position of the pure Fn spot is indicated, and the box shows its nearest extracellular matrix (ECM) neighbors. (B) Diagram showing pattern of protein deposition for each of the six ECM proteins used to print the microarray. (C) Proteins were deposited in four replicates onto HydroGel microarray slides using a Virtek ChipWriter Pro robotic microarrayer.

Article Snippet: Four replicates of each protein mixture were deposited on the Hydrogel surface using a Virtek ChipWriter Pro (BioRad, Hercules, CA) robotic microarrayer equipped with Stealth SMP 2.0 split pins (TeleChem International, Sunnyvale, CA).

Techniques: Microarray